In this study human skin fibroblasts were incubated without or with increasing concentrations of Enzogenol and the intracellular load of hydrogen peroxide produced by the cells was determined.
Research on external oxidative stress has shown that Enzogenol can protect skin cells from the harmful effects of free radicals present in the environment.
In this study Enzogenol was compared to Vitamin C for its ability to protect human skin fibroblast growth in the presence of hydrogenperoxide [H2O2 leads to cell death and growth depression]. Compared to the H2O2-treated control cells [set to 100%] the cultures with Enzogenol showed growth of up to 132%, whereas Vitamin C failed to protect the cells.
Research shows that Enzogenol can protect skin cells from the harmful effects of UV-radiation.
Here human skin fibroblasts were treated with UV-radiation. UV led to growth depression of 28-52% in the controls, whereas the Enzogenol protected cells showed much lower growth depression of 8-29%.
Proanthocyanidins bind to and stabilize collagen.
The PAs from the pinus radiata bark that make up 80% of Enzogenol can bind to and stabilize collagen, with the highest adsorption capacity between pH 4-7 covering the natural pH level of the skin (5.5-6.5). PA binding protects collagen from digestion/solubilization by collagenase. (Ku, C. S. et al Chemosphere 67: 1618-1627; 2007. Han, B. et al J Biomed Mater Res A 65A: 118-124; 2003.)