Skip to menu

Supporting Skin Health
In addition to the health support of the Enzogenol pine bark extract as a food or dietary supplement, there are several functional applications for topical use in personal care formulations.

These applications are based on skin cell culture studies demonstrating that Enzogenol:
- Penetrates the skin
- Boosts the skin cells natural internal free radical defense
- Stimulates skin cell growth
- Protects the skin cells from external free radical damage
- Protects skin cells from damaging UV radiation
- Binds and protects collagen

This research was carried out by Dr. Paul Davies of the Bioactivities Invesigation Group, Otago School of Medicine, Wellington, New Zealand.

Research has shown that the proanthocyanidin (PA) compounds in Enzogenol can penetrate the skin.



In this study a 1% Enzogenol solution (in 10% glycerin) was applied to the outside of a sample of rat skin; while the inside of the skin was in contact with a buffer solution. Liquid samples were taken periodically from the inside buffer and were assayed by HPLC for the presence of catechin and polymeric PAs that had penetrated across the skin.


Research has demonstrated that Enzogenol can stimulate skin cell growth.






Here human skin fibroblasts were incubated with Enzogenol added to the growth medium at 1 μg/ml. In comparison to untreated cells Enzogenol stimulated cell growth by up to 10%.

Research on internal oxidative stress has shown that Enzogenol can protect skin cells from the free radicals that are produced internally through the normal skin metabolism.




In this study human skin fibroblasts were incubated without or with increasing concentrations of Enzogenol and the intracellular load of hydrogen peroxide produced by the cells was determined.


Research on external oxidative stress has shown that Enzogenol can protect skin cells from the harmful effects of free radicals present in the environment.






In this study Enzogenol was compared to Vitamin C for its ability to protect human skin fibroblast growth in the presence of hydrogenperoxide [H2O2 leads to cell death and growth depression]. Compared to the H2O2-treated control cells [set to 100%] the cultures with Enzogenol showed growth of up to 132%, whereas Vitamin C failed to protect the cells.

Research shows that Enzogenol can protect skin cells from the harmful effects of UV-radiation.




Here human skin fibroblasts were treated with UV-radiation. UV led to growth depression of 28-52% in the controls, whereas the Enzogenol protected cells showed much lower growth depression of 8-29%.

Proanthocyanidins bind to and stabilize collagen.

The PAs from the pinus radiata bark that make up 80% of Enzogenol can bind to and stabilize collagen, with the highest adsorption capacity between pH 4-7 covering the natural pH level of the skin (5.5-6.5). PA binding protects collagen from digestion/solubilization by collagenase. (Ku, C. S. et al Chemosphere 67: 1618-1627; 2007. Han, B. et al J Biomed Mater Res A 65A: 118-124; 2003.)